NF-1B2 antibody (pAb)

RRID: AB_2314934
Aliases: NFIB, CTF, NF1-B, NFIB2, NFIB3, NF-I/B, NFI-RED, HMGIC/NFIB
Catalog No: 39091 Format: 100 µg Discontinued

Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
50 kDa
Reactivity:
Human, Rat

Applications

ChIP Validated Western Blot Validated Immunofluorescence Validated Electrophoretic Mobility Shift Assay Validated

Application Notes

This product has been discontinued:
b>Applications Validated by Active Motif:
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP
WB
IF
EMSA

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This NF-1B2 antibody was raised against a synthetic peptide corresponding to amino acid residues 402-415 of human NF-1B2.

Buffer

Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

NF-1B2 (NF-1, Nuclear factor-1) is a ubiquitous 47 kDa dimeric DNA-binding protein whose recognition sequence, TGG(C/A)N(5)GCCAA, is found in the genomes of a number of DNA viruses. The same sequence occurs frequently in the human genome. The NF-1 protein stimulates initiation of adenovirus DNA replication in vitro and is capable of stimulating the transcription of genes in cooperation with other factors, such as the estrogen receptor. The Nuclear factor-1 (NF-1) family of transcription factors are encoded by the genes NF-1A, NF-1B2, NF-1C and NF-1X and feature a highly conserved N-terminal DNA binding region and a variable C-terminal transcriptional activation or repression domain. NF-1 binding controls the expression of many target genes, including insulin, TGF-β, nucleotide, steroid hormone and vitamin synthetic genes, TNFα and FSH. NF-1 transcripts can be differentially spliced and the resultant protein variants homo- and heterodimerize, allowing for multiple potential transcriptional activation and repression activities.

Positive Control

Active Motif's Rat Liver nuclear extract (Catalog No. 36024) can be used as a positive control for NF-1B2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with NF-1B2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting