Copine I antibody (pAb)

Aliases: CPNE1, CPN1, COPN1
Catalog No: 39101 Format: 200 µl $435 Buy
Catalog No: 39102 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
68 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Published Applications:
WB
See references for more information. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. 

Immunogen

This Copine I antibody was raised against a peptide corresponding to amino acids 519-532 of human Copine I.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Copine I antibody (pAb) tested by Western blot.

Copine I pAb tested by Western blot.
Detection of Copine I by Western blot analysis. 20 µg of PC-3 whole-cell extract probed with Copine I pAb at a 1:2,000 dilution.

Background

Copine I (CPNE1) is a member of a family of calcium phospholipid-binding proteins that play a role in cell signaling. Copine I augments the activated Tumor Necrosis Factor-alpha (TNF-α) signaling pathway in a calcium-dependent manner. Copine I is a repressor protein that physically interacts with NFκB p65 to inhibit NFκB-mediated transcription.

Positive Control

Active Motif's PC-3 nuclear extract (Catalog No. 36082) can be used as a positive control for Copine I antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting