KAT7 / Hbo1 antibody (pAb)

RRID: AB_2793156
Aliases: KAT7, HBOA, MYST2, ZC2HC7
Catalog No: 39105 Format: 200 µl $460 Buy
Catalog No: 39106 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
75 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts.

Immunogen

This Hbo1 antibody was raised against a synthetic peptide containing amino acids 158-172 of human Hbo1.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Hbo1 pAb tested by Western blot.
Detection of Hbo1 by Western blot analysis. HeLa whole-cell extract (15 µg) was probed with Hbo1 pAb at a 1:500 dilution.

Background

Hbo1 (HAT binding to ORC1) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, Hbo1 is thought to participate in events controlling the initiation of DNA replication.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Hbo1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting