Hbo1 antibody (pAb)

Aliases: KAT7, HBOA, MYST2, ZC2HC7
Catalog No: 39105 Format: 200 µl $435 Buy
Catalog No: 39106 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
75 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

For optimal results, primary antibody incubations should be performed overnight at 4°C. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts. 

Immunogen

This Hbo1 antibody was raised against a synthetic peptide containing amino acids 158-172 of human Hbo1.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Hbo1 antibody (pAb) tested by Western blot.

Hbo1 pAb tested by Western blot.
Detection of Hbo1 by Western blot analysis. HeLa whole-cell extract (15 µg) was probed with Hbo1 pAb at a 1:500 dilution.

Background

Hbo1 (HAT binding to ORC1) is a MYST-family histone acetyltransferase (HAT) that forms a complex with ING4/5, JADE1/2/3, and Eaf6, which is found enriched on coding regions of genes, supporting a role in transcriptional elongation. In addition, Hbo1 is thought to participate in events controlling the initiation of DNA replication.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Hbo1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting