Histone H2AK9ac antibody (pAb)

Catalog No: 39109 Format: 200 µl $435 Buy
Catalog No: 39110 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
14 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated Immunofluorescence Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:2,000 - 1:5,000 dilution
IF: 1:500 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts.

Immunogen

This Histone H2A acetyl Lys9 antibody was raised against a peptide including acetyl-lysine 9 of human histone H2A.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Histone H2AK9ac antibody (pAb) tested by Western blot.

Histone H2A acetyl Lys9 pAb tested by Western blot.
Western blot probed with Histone H2A acetyl Lys9 polyclonal antibody (1:5,000 dilution).
    Lane 1: 20 ng recombinant histone H2A.
    Lane 2: 5 µg acid extract of HeLa cells.
    Lane 3: 5 µg acid extract of HeLa cells treated with sodium butyrate.

Histone H2AK9ac antibody (pAb) tested by dot blot analysis.

Specificity data:
Dot blot analysis was used to confirm the specificity of Histone H2A acetyl Lys9 pAb for acetyl Lys9 of Histone H2A. Modified and unmodified peptides were spotted onto PVDF and probed with the antibody at a 1:5,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
    Lane 1: Peptide acetylated at Lys5 of H2A.
    Lane 2: Unmodified Lys5 peptide.
    Lane 3: Peptide acetylated at Lys9 of H2A.
    Lane 4: Unmodified Lys9 peptide.

Histone H2AK9ac antibody (pAb) tested by immunofluorescence.

Detection of H2AK9ac by immunofluorescence.
U2OS cells were stained with H2AK9ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2AK9ac antibody staining. Right panel: merge.

Background

Histone H2A is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

Lysine N-ε-acetylation is a dynamic, reversible and tightly regulated protein and histone modification that plays a major role in chromatin remodeling and in the regulation of gene expression in various cellular functions. Histone H2A and Histone H2B are acetylated in bulk chromatin by p300 and form acetylated H2A/H2B heterodimers. When DNA associates with intact core histone octamers that contain acetylated H2A/H2B heterodimers, the inhibition of transcriptional initiation significantly decreases, indicating that acetylation of their lysine residues may mediate transcription.

Positive Control

Active Motif's HeLa acid extract (Sodium butyrate treated) (Catalog No. 36202) can be used as a positive control for Histone H2A acetyl Lys9 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2AK9ac antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting