Rad52 antibody (pAb)

Catalog No: 39196 Format: 200 µg $435 Buy
Catalog No: 39197 Format: 10 µg $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
46 kDa
Reactivity:
Human

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 2 - 10 µg/ml dilution

Immunogen

This Rad52 antibody was raised against full-length human Rad52 expressed in E. coli.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Rad52 antibody (pAb) tested by immunofluorescence.

Rad52 pAb tested by immunofluorescence.
Staining of HEK293 cells with Rad52 pAb at a 2 µg/ml dilution.

Background

Rad52 is important for DNA double-strand break repair and homologous recombination. Rad52 is shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. Rad52 is also found to interact with DNA recombination protein Rad51, which suggested its role in RAD51-related DNA recombination and repair.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad52 antibody.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Rad52 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting