Rad52 antibody (pAb)

RRID: AB_2793179
Catalog No: 39196 Format: 200 µg $460 Buy
Catalog No: 39197 Format: 10 µg $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein A Chromatography
Host:
Rabbit
Molecular Weight:
46 kDa
Reactivity:
Human

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 2 - 10 µg/ml dilution

Immunogen

This Rad52 antibody was raised against full-length human Rad52 expressed in E. coli.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Rad52 pAb tested by immunofluorescence.
Staining of HEK293 cells with Rad52 pAb at a 2 µg/ml dilution.

Background

Rad52 is important for DNA double-strand break repair and homologous recombination. Rad52 is shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. Rad52 is also found to interact with DNA recombination protein Rad51, which suggested its role in RAD51-related DNA recombination and repair.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad52 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting