APE antibody (pAb)

RRID: AB_2793181
Aliases: APEX1, APX, APE1, APEN, APEX, HAP1, REF1
Catalog No: 39200 Format: 100 µg $460 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
37 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This APE antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 36-52 and 216-232 of human APE.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

APE pAb tested by Western blot.
APE detection by Western blot. The analysis of APE was performed using HeLa whole-cell extract and APE pAb at a 2 µg/ml dilution.

Background

APE (apurinic endonuclease or apyrimidinic endonuclease) is a multifunctional DNA repair enzyme. The main role of APE is to create a nick in the phosphodiester backbone of the AP site that is created when DNA glycosylase removes the damaged base. Four types of APE (AP endonucleases) are classified according to their site of incision.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for APE antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting