INHAT-1/TAF-1α/TAF-1β antibody (pAb)

Aliases: TAF1A, SL1, RAFI48, TAFI48
Catalog No: 39202 Format: 200 µl $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
37 kDa
Reactivity:
Human, Mouse, Rat

Applications

Western Blot Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Published Applications:
WB
ICC

Immunogen

This INHAT-1/TAF-1a/TAF-1b antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 66-81 and 135-151 of human INHAT-1/TAF-1a/TAF-1β.

Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

References

 
INHAT-1/TAF-1α/TAF-1β antibody (pAb) tested by Western blot.

INHAT-1/TAF-1α/TAF-1β pAb tested by Western blot.
INHAT-1 detection by Western blot. The analysis was performed using Jurkat whole-cell extract and INHAT-1/TAF-1α/TAF-1β pAb at a 2 µg/ml dilution.

Background

INHAT (also designated TAF-1α, TAF-1β and inhibitor of histone acetyltransferases complex) binds to histones and masks them from being substrates for histone acetyltransferases (HATs). Endogenous INHAT subunits, which include the Set/TAF-Ibeta oncoprotein, and pp32 associate with chromatin in vivo and can block coactivator-mediated transcription when transfected in cells. Single phosphorylations within the Histone H3 tail blocks binding of INHAT, as well as simultaneous acetylation of multiple lysine residues.

Positive Control

Active Motif's Jurkat nuclear extract (Catalog No. 36014) can be used as a positive control for INHAT-1/TAF-1α/TAF-1β antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting