CXXC1 antibody (pAb)

RRID: AB_2615050
Aliases: CGBP, CFP1, SPP1, PCCX1, PHF18, hCGBP, ZCGPC1
Catalog No: 39203 Format: 200 µl $460 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Rabbit
Molecular Weight:
88 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This CXXC1 antibody was raised against a mixture of synthetic peptides corresponding to amino acid residues 73-90 and 641-656 of human CXXC1.

Buffer

PBS containing 0.05% BSA and 0.02% sodium azide. Sodium azide is highly toxic.

 

CXXC1 pAb tested by Western blot.
Detection of CXXC1 by Western blot analysis. Western blot analysis using CXXC1 pAb at a 1:1,000 dilution against 15 µg/lane of human spleen lysate.

Background

CXXC1 (also known as CGBP or CpG binding protein) is a transcriptional activator protein that binds unmethylated CpG dinucleotides and plays a role in regulating gene expression and embryonic development. CGBP exhibits a different distribution pattern than other heterochromatin-associated proteins. It seems to co-localize with acetylated histones, indicating that CXXC1 is associated with active chromatin.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting