LIG1 antibody (pAb)

Catalog No: 39214 Format: 200 µl $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
100 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This LIG1 antibody was raised against a peptide corresponding to amino acid residues 51-68 of human LIG1.

Buffer

PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.

References

 
LIG1 antibody (pAb) tested by Western blot.

LIG1 pAb tested by Western blot.
Detection of LIG1 by Western blot. The analysis was performed using HeLa nuclear extract and LIG1 pAb at a 1:1,000 dilution.

Background

LIG1 is an ATP-dependent DNA ligase that participates in base excision and repair processes during DNA replication.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for LIG1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting