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Applications
for LIG1 antibody (pAb)Application Notes
Validated Applications:
WB: 1:500 - 1:1,000 dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.
for LIG1 antibody (pAb)Immunogen
This LIG1 antibody was raised against a peptide corresponding to amino acid residues 51-68 of human LIG1.
for LIG1 antibody (pAb)Buffer
PBS containing 0.02% sodium azide and 0.1 mg/ml BSA. Sodium azide is highly toxic.
for LIG1 antibody (pAb)References
LIG1 pAb tested by Western blot.
Detection of LIG1 by Western blot. The analysis was performed using HeLa nuclear extract and LIG1 pAb at a 1:1,000 dilution.
for LIG1 antibody (pAb)Background
LIG1 is an ATP-dependent DNA ligase that participates in base excision and repair processes during DNA replication.
for LIG1 antibody (pAb)Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for LIG1 antibody.
for LIG1 antibody (pAb)Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.
for LIG1 antibody (pAb)Guarantee
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
- WB = Western Blotting
