PMS2 antibody (mAb)

Clone: 163C1251
Aliases: PMSL2, HNPCC4, PMS2CL
Catalog No: 39221 Format: 100 µg $435 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
85 kDa
Human, Mouse


Western Blot Validated

Application Notes

Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.


This PMS2 antibody was raised against a peptide corresponding to amino acid residues 623-639 of human PMS2.


PBS containing 0.02% sodium azide. Sodium azide is highly toxic.


PMS2 antibody (mAb) tested by Western blot.

PMS2 mAb tested by Western blot.
PMS2 detection by Western blot. The analysis was performed using NIH/3T3 whole-cell extract and PMS2 pAb at a 2 µg/ml dilution.


PMS2Post-meiotic Segregation Increased 2 is the human homolog of the yeast Pms2 protein and is involved in post-replicative DNA mismatch repair (MMR). It forms a heterodimer with MLH-1 and this complex interacts with other protein complexes that recognize and bind to mismatched bases. PMS2 has endonuclease activity and introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation of the existing strand prevents cleavage and dictates that only the unmethylated, newly replicated (and likely to have the incorrect base) strand be repaired. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer and Turcot syndrome.

Positive Control

Active Motif's NIH/3T3 nuclear extract (Catalog No. 36020) can be used as a positive control for PMS2 antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting