Rad17 phospho Ser647 antibody (pAb)

Aliases: CCYC, R24L, RAD24, HRAD17, RAD17SP
Catalog No: 39222 Format: 100 µg $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
77 kDa
Reactivity:
Human, Rat

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.1 - 1 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This Rad17 phospho Ser647 antibody was raised against a synthetic peptide corresponding to mouse Rad17 phosphorylated at serine 647 .

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
Rad17 phospho Ser647 antibody (pAb) tested by Western blot.

Rad17 phospho Ser647 pAb tested by Western blot.
Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B).

Background

Rad17 Ser647 (Rad24, CCYC, R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.

Positive Control

A recombinant Rad17 protein containing the phosphorylated Ser647 residue can be used as a positive control for Rad17 phospho Ser647 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting