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Rad17 phospho Ser647 antibody (pAb)
| RRID: AB_2793192 |
| Aliases: CCYC, R24L, RAD24, HRAD17, RAD17SP |
| Catalog No: 39222 | Format: 100 µg | $480 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
WB: 0.1 - 1 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Immunogen
This Rad17 phospho Ser647 antibody was raised against a synthetic peptide corresponding to mouse Rad17 phosphorylated at serine 647 .
Buffer
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Rad17 phospho Ser647 pAb tested by Western blot.
Rad17 detection by Western blot. The analysis of phospho-RAD17 was performed using the Rad17 phospho Ser647 pAb and partial recombinant Rad17 protein containing phospho Ser647 (lane A) and the same sequence without a phospho serine (lane B).
Background
Rad17 Ser647 (Rad24, CCYC, R24L) is essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 has a weak ATPase activity required for binding to chromatin. Rad17 participates in the recruitment of the Rad1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. Rad17 may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination.
Positive Control
A recombinant Rad17 protein containing the phosphorylated Ser647 residue can be used as a positive control for Rad17 phospho Ser647 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
