UBE2V1α antibody (pAb)

RRID: AB_2793195
Aliases: CIR1, UEV1, CROC1, UBE2V, UEV-1, UEV1A, CROC-1
Catalog No: 39225 Format: 100 µg $460 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
20 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 3 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This UBE2V1a antibody was raised against a peptide corresponding to amino acid residues 31-49 of human UBE2V1a.

Buffer

PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic.

 

UBE2V1α pAb tested by Western blot.
Detection of UBE2V1α by Western blot. The analysis was performed using HeLa whole-cell extract and UBE2V1α pAb at a 2 µg/ml dilution.

Background

UBE2N and its variants UBE2V1α and β are members of the E2 ubiquitin-conjugating enzyme family, and the homologs of yeast UBC13. The UBE2V1/UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys63. This type of poly-ubiquitination activates IKK (IKKα, IKKβ & IKKγ) and does not seem to involve protein degradation by the proteasome. UBE2V1 plays a role in the activation of NFκB (NFκB p50 & NFκB p65) mediated by IL-1β, TNF, TRAF6 and TRAF2.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for UBE2V1α antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting