Jhd2 antibody (pAb)

Aliases: Lysine Demethylase 5C, Histone Demethylase JARID1C, KDM5C
Catalog No: 39263 Format: 200 µl $435 Buy
Catalog No: 39264 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
85 kDa
Reactivity:
Budding Yeast

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution

Published Applications:

WB

Immunogen

This Jhd2 antibody was raised against a peptide corresponding to the C-terminus of Jhd2 from S. cerevisiae.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol, 0.7 mg/ml BSA and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Jhd2 antibody (pAb) tested by Western blot.

Jhd2 pAb tested by Western blot.
S. cerevesiae extracts probed with Jhd2 pAb (1:1,000 dilution).
   Lane 1: wild type strain.
   Lane 2: Jhd2 knockout strain.

Background

Jhd2 is a JmjC domain family histone demethylase specific for H3K4 (lysine at position 4 of the histone H3 protein). It removes methyl groups specifically added by Set1p methyltransferase.

Positive Control

Whole-cell extracts from yeast can be used as positive control for Jhd2 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting