Mi-2 beta antibody (pAb)
|Aliases: CHD4, Mi-2b|
|Catalog No: 39289||Format: 100 µl|
|Catalog No: 39290||Format: 10 µl|
This product has been discontinued:
Please refer to our alternative product Catalog No. 39695
IHC (FFPE): 1:100
ICC/IF: 1:500 - 1:2,000 dilution
WB: 1:500 - 1:1,000 dilution
For Mi-2 beta (CHD4), we also offer AbFlex® Mi-2 beta (CHD4) Recombinant Antibody (rAb). For details, see Catalog No. 91275.
This Mi-2B antibody was raised against a recombinant protein corresponding to amino acids 22-155 of mouse Mi-2 beta.
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Mi-2β (Mi-2beta, CHD4, chromodomain helicase DNA-binding protein 4) represents the major Mi-2 isoform present in the complex Mi-2/NuRD. This complex is a multi-subunit protein complex containing both histone deacetylase and nucleosome-dependent ATPase subunits. Current models predict that this complex functions primarily in transcriptional repression. In addition to the ATPase domain, Mi-2 beta contains a chromodomain and two PHD domains. Mi-2 beta interacts with BRG-1, a component of the SWI/SNF complex, and the RET finger protein (RFP).
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Mi-2 beta antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- WB = Western Blotting