Aiolos antibody (pAb)

RRID: AB_2615037
Aliases: IKZF3, AIO, ZNFN1A3
Catalog No: 39293 Format: 200 µl $445 Buy
Catalog No: 39294 Format: 10 µl $110 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
58 kDa
Reactivity:
Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 dilution

The protein migrates around 70 kDa, and multiple isoforms may be detected due to alternative splicing as seen in the example Western blot image.

Published Applications:
Immunoprecipitation (IP)

Immunogen

This Aiolos antibody was raised against a recombinant GST-Tagged protein corresponding to the C-terminal half of mouse Aiolos.

Buffer

Rabbit antisera with 30% glycerol containing 0.035% sodium azide. Sodium azide is highly toxic.

References

 

Aiolos pAb tested by Western blot.
Detection of Aiolos by Western blot analysis. Aiolos was detected using a 1:1,000 dilution of Aiolos pAb with 20 µg mouse spleen tissue extract.

Background

Aiolos transcription factor is a member of the Ikaros family of zinc-finger proteins. Aiolos is a hemopoietic-specific transcription factor that regulates B lymphocyte proliferation and differentiation. It can also participate in chromatin remodeling.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting