Histone H3K122me1 antibody (pAb)

Catalog No: 39367 Format: 200 µl $435 Buy
Catalog No: 39368 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Other (Wide Range)

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:250 - 1:1,000 dilution

Published Applications: see references

Immunogen

This Histone H3 monomethyl Lys122 antibody was raised against a peptide containing monomethyl-lysine 122 of human histone H3.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Histone H3K122me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Lys122 pAb tested by Western blot.
Nuclear extract of HeLa cells (20 µg) was probed with Histone H3 monomethyl Lys122 pAb (1:500 dilution).

Histone H3K122me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Lys122 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of 39367 for monomethyl Lys122 histone H3. Methylated peptides corresponding to the immunogen were spotted onto PVDF and probed with 39367 at 1:5,000. The amount of peptide (picomoles) spotted is indicated next to each row.
    Lane 1: unmodified Lys122 H3 peptide.
    Lane 2: monomethyl-Lys122 H3 peptide.
    Lane 3: dimethyl-Lys122 H3 peptide.
    Lane 4: trimethyl-Lys122 H3 peptide.

Background

Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression.

The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3 monomethyl Lys122 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting