NBS1 antibody (pAb)

for NBS1 antibody (pAb)Application Notes

Validated Applications:
WB: 1:500 - 1:1,000 dilution

for NBS1 antibody (pAb)Immunogen

This NBS1 antibody was raised against a peptide containing to the C-terminus of human NBS1.

for NBS1 antibody (pAb)Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

for NBS1 antibody (pAb)References

for NBS1 antibody (pAb)Background

NBS1 (Nijmegen breakage syndrome 1, NBN or p95) is a component of the MRE11/RAD50/NBN (MRN complex), which plays a critical role in the cellular response to DNA damage and the maintenance of chromosome integrity. The complex is involved in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity, cell cycle checkpoint control and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3′-5′ exonuclease activity, which are provided by MRE11A. NBN modulate the DNA damage signal sensing by recruiting PI3-kinase/PI4-kinase family members ATM, ATR, and probably DNA-PKcs to the DNA damage sites and activating their functions. NBS1 (NBN) also functions in telomere length maintenance by generating the 3′ overhang which serves as a primer for telomerase dependent telomere elongation. NBS1 (NBN) is a major player in the control of intra-S-phase checkpoint and there is some evidence that NBS1 (NBN) is involved in G₁ and G₂ checkpoints. The roles of NBS1 (NBN)/MRN encompass DNA damage sensor, signal transducer, and effector, which enable cells to maintain DNA integrity and genomic stability.

for NBS1 antibody (pAb)Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for NBS1 antibody.

for NBS1 antibody (pAb)Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

for NBS1 antibody (pAb)Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.