Bcl10 antibody (mAb)

Clone: 151
Aliases: CLAP, mE10, CIPER, c-E10, CARMEN
Catalog No: 39393 Format: 200 µg $435 Buy
Catalog No: 39394 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
33 kDa
Reactivity:
Human

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µg per IP
WB: 0.5 - 2 µg/ml dilution

Immunogen

This Bcl10 antibody was raised against full-length recombinant human Bcl10 protein.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Bcl10 antibody (mAb) tested by immunoprecipitation.

Bcl10 antibody (mAb) (Clone 151) tested by Immunoprecipitation.
10 µg of Bcl10 antibody was used to immunoprecipitate Bcl10 from 500 µg of K562 whole cell extract (lane 2). 10 µg of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the Bcl10 antibody at a 1 µg/ml dilution.

Bcl10 antibody (mAb) tested by Western blot.

Bcl10 antibody (mAb) (Clone 151) tested by Western blot.
Bcl10 detection by Western blot. The analysis was performed using 20 µg HeLa nuclear extract and Bcl10 mAb (Clone 151) at a 1 µg/ml dilution.

Background

Bcl10 (B-cell CLL/lymphoma 10) protein promotes apoptosis and activation of NFκB (NFκB p50 and NFκB p65) via NIK and IKK (IKKα, IKKβ, IKKi/IKKε and IKKγ). Bcl10 protein contains a caspase recruitment domain (CARD) which interacts with other CARD domain containing proteins to regulate NFκB signaling. It is also a substrate for MALT1.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Bcl10 antibody (Clone 151).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use Bcl10 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting