SMARCA5 antibody (mAb)

Aliases: SNF2H, SWI/SNF, ISWI, hISWI, hSNF2H, WCRF135
Catalog No: 39543 Format: 200 µl $435 Buy
Catalog No: 39544 Format: 10 µl $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Ascites
Host:
Mouse
Molecular Weight:
135 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:2,000 dilution
WB: 1:500 - 1:2,000 dilution

Published Applications:
ChIP
IP
See references for more information. Individual optimization may be required.

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This antibody was raised against purified human RSF complex which was used to immunize mice.

Buffer

Ascites containing 30% glycerol and 0.035% sodium azide.

References

 
SMARCA5 antibody (mAb) tested by immunofluorescence.

SMARCA5 antibody (mAb) tested by Immunofluorescence.
Formaldehyde fixed HeLa cells stained with SMARCA5 antibody at a 1:2,000 dilution.

SMARCA5 antibody (mAb) tested by Western blot.

SMARCA5 antibody (mAb) tested by Western blot.
HeLa acid extract (10 µg) was probed with SMARCA5 mAb (1:2,000 dilution).

Background

SMARCA5 is a human homolog of the budding yeast Snf2 protein. SMARCA5 is the catalytic subunit of several chromatin remodeling complexes, such as CHRAC, RSF, ACF, NuRD and NoRC. SMARCA5 is a member of the SWI/SNF superfamily of proteins and members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. SMARCA5 is a component of the and spacing factor RSF (Rsf1), a facilitator of the transcription of class II genes by RNA pol II. It is similar in sequence to the Drosophila ISWI chromatin remodeling protein. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein.

Positive Control

Active Motif's HeLa acid extract (Catalog No. 36200) can be used as a positive control for SMARCA5 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SMARCA5 antibody (mAb).

Bridging Antibody to Improve ChIP, MeDIP and IP

Some isotypes of mouse IgG antibodies do not bind well to protein G-conjugated agarose/magnetic beads, which are commonly used in ChIP, MeDIP and IP experiments. Capture efficiency can be improved by including an anti-mouse IgG bridging antibody because it binds with a strong affinity to both the protein G beads and the mouse IgG antibody. By increasing the affinity of the mouse primary antibody for the protein G bead, the Bridging Antibody for Mouse IgG can help improve the results of all ChIP, MeDIP and IP experiments that use SMARCA5 antibody (mAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with SMARCA5 antibody (mAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting