STAT2 phospho Tyr689 antibody (pAb)

Aliases: P113, ISGF-3, STAT113
Catalog No: 39611 Format: 200 µl $435 Buy
Catalog No: 39612 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
110 kDa
Reactivity:
Human

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Immunogen

This STAT2 phospho Tyr689 antibody was raised against a peptide containing phospho Tyr689 of human STAT2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
STAT2 phospho Tyr689 antibody (pAb) tested by Western blot.

STAT2 phospho Tyr689 pAb tested by Western blot.
HeLa whole-cell extract (20 µg per lane) was probed with STAT2 phospho Tyr689 pAb (1:500).
    Lane 1: No treatment.
    Lane 2: Cells treated with IFNα (2500 units/ml for 30 minutes).

STAT2 phospho Tyr689 antibody (pAb) tested by dot blot analysis.

STAT2 phospho Tyr689 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of STAT2 phospho Tyr689 pAb for STAT2 phospho Tyr689. Phosphorylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at 1:10,000. The amount of peptide (picomoles) spotted is indicated next to each row.
Lane 1: Unmodified Ser727 STAT1 peptide. Lane 2: Phospho Ser727 STAT1 peptide. Lane 3: Unmodified Tyr689 STAT2 peptide. Lane 4: Phospho Tyr689 STAT2 peptide. Lane 5: Unmodified Ser727 STAT3 peptide. Lane 6: Phospho Ser727 STAT3 peptide. Lane 7: Unmodified Tyr705 STAT3 peptide. Lane 8: Phospho Tyr705 STAT3 peptide. Lane 9: Unmodified Ser726 STAT5A/Ser731 STAT5B peptide. Lane 10: Phospho Ser726 STAT5A/Ser731 STAT5B peptide. Lane 11: Unmodified Tyr694 STAT5A/Tyr699 STAT5B peptide. Lane 12: Phospho Tyr694 STAT5A/Tyr699 STAT5B peptide.

Background

STAT2 (signal transducer and activator of transcription 2) is a member of the STAT family of transcription factors. STATs undergo tyrosine and serine phosphorylation in response to growth factor or cytokine signaling. This phosphorylation results in dimerization and translocation of STAT proteins to the nucleus. STAT2 has been shown to be activated by IFNα and IFNβ, and to associate with the transcription factors p48 and p300.

Positive Control

Whole-cell extracts from cells treated with IFN-alpha (2500 units/ml for 30 minutes) can be used as positive control for STAT2 phospho Tyr689 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting