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HMGA1 antibody (pAb)
| Aliases: HMG-R, HMGIY, HMGA1A |
| Catalog No: 39615 | Format: 100 µl | $500 | Buy |
| Catalog No: 39650 | Format: 10 µl | $125 | Buy |
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Applications
Application Notes
Applications Validated by Active Motif:
ICC/IF: 1:1,000 dilution
WB: 1:500 - 1:2,000 dilution
Published Applications
The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:
WB
View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this product.
Immunogen
This HMGA1 antibody was raised against a peptide corresponding to the N-terminus of human HMGA1.
Buffer
Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
HMGA1 antibody tested by immunofluorescence.
Detection of HMGA1 by immunofluorescence. HeLa cells were stained with HMGA1 antibody at a dilution of 1:1,000. Top panel: HMGA1 antibody staining. Middle panel: DAPI. Bottom panel: merge.
HMGA1 antibody tested by Western blot.
HeLa nuclear extract (20 µg per lane) was probed with HMGA1 pAb (1:500 dilution). Lane 1: no treatment.
Background
High mobility group (HMG) proteins are nuclear proteins that may significantly affect DNA interactions by altering double-stranded DNA structure. HMGA1 is frequently acetylated and is found in the nucleus.
Positive Control
Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for HMGA1 antibody.
Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
Guarantee
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
Application Key
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting
