HA-Tag antibody (mAb)

RRID: AB_2793281
Clone: 3B9
Catalog No: 39627 Format: 200 µg $460 Buy
Catalog No: 39628 Format: 10 µg $115 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
N/A
Reactivity:
Human, Not Species Specific

Applications

Immunoprecipitation Validated

Application Notes

Applications Validated by Active Motif:
IP: 2 - 5 µg per IP

This antibody is also available as an AbFlex® engineered recombinant antibody. For details on the corresponding AbFlex Recombinant Antibody, see Catalog No. 91271.

Immunogen

This HA-Tag antibody was raised against a peptide containing multiple repeats of the HA-Tag epitope, YPYDVPDYA.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

HA-Tag mAb tested by IP / Western blot.
Whole-cell lysate (400 µg) from U2OS cells expressing an HA-Tagged protein was immunoprecipitated with 2 µg of either control mouse IgG (lane 1) or HA-Tag mAb (lane 2). Immunoprecipitates were run on an SDS-PAGE gel and analyzed by Western blot using a second HA-Tag antibody.

Background

HA-Tag has been derived from the hemagglutinin protein of the human influenza virus for use as an epitope for the detection and purification of tagged recombinant proteins expressed in vivo. The HA-Tag is a small epitope and generally does not interfere with the normal function of recombinant proteins.

Positive Control

Immunoprecipitated extracts from U2OS cells expressing a HA-Tagged protein can be used as a control for HA Tag antibody (Clone 3B9).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting