Pht1 / Histone H2A.Z antibody (pAb)

RRID: AB_2793287
Catalog No: 39640 Format: 100 µl $460 Buy
Catalog No: 39641 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
15 kDa
Reactivity:
Fission Yeast

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

ChIP-qPCR
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This Pht1 / H2AZ antibody was raised against a peptide corresponding to the C-terminus of Pht1, the histone H2A variant in S. pombe.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

Pht1 / H2AZ pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ pAb (1:500 dilution).

Background

Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.

Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting