Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb)

RRID: AB_2793288
Catalog No: 39642 Format: 100 µl $460 Buy
Catalog No: 39643 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
15 kDa
Reactivity:
Fission Yeast, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 - 1:1,000 dilution

Immunogen

This Pht1 / H2AZ acetyl antibody was raised against a peptide containing acetyl-lysine at positions 5, 7, 12 and 16 from the amino terminus of Pht1, the histone H2AZ variant in S. pombe.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

 

Pht1 / H2AZ acetyl pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ acetyl pAb (1:500 dilution).

Pht1 / H2AZ acetyl pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Pht1 / H2AZ acetyl pAb for acetylation of Pht1, the S.pombe variant for Histone H2AZ. Modified and unmodified peptides were spotted onto PVDF and probed with Pht1 / H2AZ acetyl pAb at a 1:1,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
   Lane 1: Peptide of acetylated Pht1
   Lane 2: Unmodified Pht1 peptide

Background

Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.

Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ acetyl antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting