Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb)

Catalog No: 39642 Format: 100 µl $435 Buy
Catalog No: 39643 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
15 kDa
Reactivity:
Fission Yeast

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:1,000 dilution

Immunogen

This Pht1 / H2AZ acetyl antibody was raised against a peptide containing acetyl-lysine at positions 5, 7, 12 and 16 from the amino terminus of Pht1, the histone H2AZ variant in S. pombe.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb) tested by Western blot.

Pht1 / H2AZ acetyl pAb tested by Western blot.
S. pombe whole-cell extract probed with Pht1 / H2AZ acetyl pAb (1:500 dilution).

Pht1 / Histone H2A.Zac (pan-acetyl) antibody (pAb) tested by dot blot analysis.

Pht1 / H2AZ acetyl pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Pht1 / H2AZ acetyl pAb for acetylation of Pht1, the S.pombe variant for Histone H2AZ. Modified and unmodified peptides were spotted onto PVDF and probed with Pht1 / H2AZ acetyl pAb at a 1:1,000 dilution. The amount of peptide spotted (in picomoles) is indicated next to each row.
   Lane 1: Peptide of acetylated Pht1
   Lane 2: Unmodified Pht1 peptide

Background

Pht1 is the S. pombe homolog of histone H2AZ and is involved in transcriptional regulation and chromosome architecture. It is also implicated in suppressing the accumulation of anti-sense transcripts through interaction with RNAi machinery and heterochromatin proteins.

Positive Control

Whole-cell extracts from S. pombe can be used as a positive control for Pht1 / H2AZ acetyl antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting