Histone H2BK12ac antibody (pAb)

Catalog No: 39669 Format: 100 µl $435 Buy
Catalog No: 39670 Format: 10 µl $105 Buy

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Antibody Type:
Molecular Weight:
15 kDa
Human, Other (Wide Range)


ChIP Validated Western Blot Validated Immunofluorescence Validated Dot Blot Validated

Application Notes

Validated Applications:
ChIP: 10 µl per ChIP
WB: 1:5,000 - 1:25,000 dilution
IF: 1:500 dilution


This Histone H2B acetyl Lys12 antibody was raised against a peptide containing acetyl Lys12 of Histone H2B.


Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.


Histone H2BK12ac antibody (pAb) tested by ChIP.

Histone H2B acetyl Lys12 pAb tested by ChIP analysis.
Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 10 µl of Histone H2B acetyl Lys12 pAb or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the PABPC1 gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

Histone H2BK12ac antibody (pAb) tested by Western blot.

Histone H2B acetyl Lys12 pAb tested by Western blot.
A549 nuclear extract (10 µg per lane) probed with Histone H2B acetyl Lys12 pAb (1:5,000 dilution). Lane 1: untreated cells. Lane 2: cells treated with Trichostatin A.

Histone H2BK12ac antibody (pAb) tested by dot blot analysis.

Histone H2B acetyl Lys12 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H2B acetyl Lys12 pAb for acetyl-Lys12 of histone H2B. Modified and unmodified peptides were spotted onto PVDF and probed with Histone H2B acetyl Lys12 pAb at a dilution of 1:5,000. The amount of peptide (in picomoles) spotted is indicated next to each row.

Lane 1: unmodified lysine 12 peptide.
Lane 2: acetyl-lysine 5 peptide.
Lane 3: acetyl-lysine 12 peptide.
Lane 4: acetyl-lysine 16 peptide.
Lane 5: acetyl-lysine 46 peptide.
Lane 6: acetyl-lysine 120 peptide.

Histone H2BK12ac antibody (pAb) tested by immunofluorescence.

Detection of H2BK12ac by immunofluorescence.
U2OS cells were stained with H2BK12ac antibody at a dilution of 1:500. Left panel: DAPI. Middle panel: H2BK12ac antibody staining. Right panel: merge.


Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. Acetylation of histones is linked to a number of specific processes including transcriptional regulation and genomic organization.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H2BK12ac antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with Histone H2BK12ac antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with Histone H2BK12ac antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.


This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting