Histone H3R8me1 antibody (pAb)

Catalog No: 39673 Format: 100 µl $435 Buy
Catalog No: 39674 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
17 kDa
Reactivity:
Human, Wide Range Predicted

Applications

Western Blot Validated Dot Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This Histone H3 monomethyl Arg8 antibody was raised against a peptide containing monomethyl-Arg8 of human histone H3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

View our Guide to Histone Modifications and Biological Function.

References

 
Histone H3R8me1 antibody (pAb) tested by Western blot.

Histone H3 monomethyl Arg8 pAb tested by Western blot.
HeLa cell nuclear extract (20 µg) probed with Histone H3 monomethyl Arg8 pAb (1:500 dilution).

Histone H3R8me1 antibody (pAb) tested by dot blot analysis.

Histone H3 monomethyl Arg8 pAb tested by dot blot analysis.
Dot blot analysis was used to confirm the specificity of Histone H3 monomethyl Arg8 pAb for monomethyl-arginine 8 of histone H3. Peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with Histone H3 monomethyl Arg8 pAb at 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row.

Top Panel:
Lane 1: unmodified Arg2 peptide.
Lane 2: monomethyl-Arg2 H3 peptide.
Lane 3: dimethyl-Arg2 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg2 H3 (asymmetric) peptide.
Lane 5: unmodified Arg8 peptide.
Lane 6: monomethyl-Arg8 H3 peptide.
Lane 7: dimethyl-Arg8 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg8 H3 (asymmetric) peptide.

Bottom Panel:
Lane 1: unmodified Arg17 H3 peptide.
Lane 2: monomethyl-Arg17 peptide.
Lane 3: dimethyl-Arg17 H3 (symmetric) peptide.
Lane 4: dimethyl-Arg17 H3 (asymmetric) peptide.
Lane 5: unmodified Arg26 peptide.
Lane 6: monomethyl-Arg26 H3 peptide.
Lane 7: dimethyl-Arg26 H3 (symmetric) peptide.
Lane 8: dimethyl-Arg26 H3 (asymmetric) peptide.

Background

Histone H3 is one of the core components of the nucleosome, the basic building block of chromatin. Histones are subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone H3 Arg8, methylated by PRMT enzymes, is involved in nuclear-receptor-mediated transcriptional activation.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for Histone H3R8me1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting