CID antibody (pAb)

Aliases: CenH3, cid, BcDNA:RE21270, cenH3, CenH3/CID, CenH3[Cid], CenH3[CID], Cenp-A, CENP-A, CENP-A/Cid, CENP-A/CID, CENP-C, cenpA, CenpA, CENPA, CenpA/CID, CG13329, Cid, CID/CENP-A, Cid[Mel], Dmel\CG13329
Catalog No: 39719 Format: 100 µl $435 Buy
Catalog No: 39720 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
30 kDa
Reactivity:
Drosophila

Applications

Immunofluorescence Validated Immunocytochemistry Validated

Application Notes


Validated Applications:
ICC/IF: 1:500 dilution

For an antibody against CID that works in Western blotting, use Catalog No. 39713.

Immunogen

This CID antibody was raised against recombinant CID protein from Drosophila melanogaster.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
CID antibody (pAb) tested by immunofluorescence.

CID pAb tested by immunofluorescence.
DrosophilaSchneider L2 cells stained with CID pAb (1:500 dilution).

Background

CID (Centromer Identifier) is the Drosophila histone H3 variant which replaces canonical H3 in centromeric chromatin at the inner plate of the kinetochore (similar to mammalian CENP-A). CID is required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. It may serve as an epigenetic mark that propagates centromere identity through DNA replication and cell division.

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with CID antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting