Acf1 antibody (pAb)

RRID: AB_2793321
Aliases: Acf, ACF, Acf-1, ACF/CHRAC, ACF1, CAF, CG1966, CHRAC, CHRAC-175, dACF, dCHRAC, Dmel\CG1966, p170/p185
Catalog No: 39721 Format: 100 µl $460 Buy
Catalog No: 39722 Format: 10 µl $115 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
185 kDa
Reactivity:
Drosophila

Applications

Western Blot Validated Immunofluorescence Validated Immunocytochemistry Validated

Application Notes

Applications Validated by Active Motif:
ICC/IF: 1:500 - 1:1,000 dilution
WB: 1:500 - 1:1,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

Immunogen

This Acf1 antibody was raised against recombinant C-terminal fragment of Acf1 from Drosophila melanogaster.

Buffer

PBS containing 0.1% sodium azide, 0.2 mg/ml BSA and 35% glycerol. Sodium azide is highly toxic.

 

Acf1 pAb tested by immunofluorescence.
Drosophila polytene chromosomes stained with Acf1 pAb (1:1,000 dilution).

Acf1 pAb tested by Western blot.
Drosophila Schneider L2 cells nuclear extract (10 µg) probed with Acf1 pAb (1:500 dilution).

Background

Drosophila Acf1 is a component of ACF, the ATP-utilizing chromatin assembly and remodeling factor protein complex. ACF catalyzes the ATP-dependent assembly of periodic nucleosome arrays in vitro. The catalytic subunit of ACF is the ISWI ATP-dependant chromatin remodeling protein. ACF is related to CHRAC (Chromatin Accessibility Complex), which also contains Acf1 and ISWI. ACF is involved in the establishment and possibly maintenance of silenced heterochromatic domains in the genome, both at pericentric heterochromatin and at genes repressed by the Polycomb complex.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting