SAP30 antibody (pAb)

RRID: AB_2561021
Aliases: Sin3A-associated protein
Catalog No: 39731 Format: 100 µl $455 Buy
Catalog No: 39732 Format: 10 µl $110 Buy

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Antibody Type:
Molecular Weight:
30 kDa


ChIP Validated ChIP-Seq Validated Western Blot Validated

Application Notes

Applications Validated by Active Motif:
ChIP: 5 µl per ChIP
ChIP-Seq & ChIP-chip: 5 µl each
WB: 1:500 dilution


This SAP30 antibody (pAb) was raised against recombinant full-length human SAP30 protein.


Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.



SAP30 antibody (pAb) tested by ChIP-Seq.
ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from the K-562 human myelogenous leukemia cell line (4.5 million cells) and 5 ul of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 30 million sequence tags were mapped to identify SAP30 binding sites. The image on the left shows SAP30 binding at transcriptional start sites across a 2.4 million bp region on chromosome 1. The image on the right is zoomed in to show SAP30 binding at two other start sites.

SAP30 antibody (pAb) tested by Western blot.
Detection of SAP30 by Western blot analysis. Lane 1: Whole cell extract of A549 cells (20 µg). Lane 2: Nuclear extract of HeLa cells (20 µg).
Both were probed with SAP30 antibody (pAb) at a 1:500 dilution.


SAP30, also known as Sin3A-associated protein, 30kDa, is part of the SIN3 corepressor complex, that also includes SIN3, SAP18, HDAC1, HDAC2, RbAp46 and RbAp48. SAP30 is involved in the functional recruitment of the Sin3-histone deacetylase complex to a specific subset of N-CoR corepressor complexes.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for SAP30 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with SAP30 antibody (pAb).


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.


This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting