Bcl3 antibody (pAb)

Aliases: B-Cell CLL/Lymphoma 3, B-Cell Lymphoma 3-Encoded Protein, BCL4, D19S37
Catalog No: 39761 Format: 100 µl $435 Buy
Catalog No: 39762 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
Serum
Purification:
None
Host:
Rabbit
Molecular Weight:
60 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This Bcl3 antibody was raised against a peptide within the C-terminal half of human Bcl3.

Buffer

Rabbit serum containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
Bcl3 antibody (pAb) tested by Western blot.

Bcl3 antibody tested by Western blot.
Detection of Bcl3 by Western blot analysis. Lane 1: HeLa whole-cell extract (20 µg). Lane 2: Nuclear extract (20 µg) of Raji cells. Both probed with Bcl3 antibody at a dilution of 1:1,000.

Background

Bcl3 (B-cell lymphoma 3 protein) functions as a transcriptional co-activator that activates through its association with NFkB homodimers. Bcl3 contains seven ankyrin repeats, which are most closely related to those found in IkB proteins. Bcl3 also functions as a form of IkB specific for the NFkB p50 subunit inhibiting its translocation to the nucleus. The expression of this gene can be induced by NFkB, which forms a part of the autoregulatory loop that controls the nuclear residence of NFkB p50. Bcl3 is overexpressed in a number of cancers, can transform fibroblasts and induce tumors in nude mice, thus implicating Bcl3 as a proto-oncogene.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Bcl3 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting