MPP8 antibody (mAb)

Clone: N119/44
Aliases: MPHOSPH8, TWA3, HSMPP8
Catalog No: 39819 Format: 100 µg $435 Buy
Catalog No: 39820 Format: 10 µg $105 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
120 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 0.5 - 2 µg/ml dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This MPP8 antibody was raised against a recombinant protein corresponding to amino acids 50-120 of human MPP8 (M-phase phosphoprotein 8).

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
MPP8 antibody (mAb) tested by Western blot.

MPP8 antibody (mAb) tested by Western blot.
Nuclear extract of HEK293 cells (20 µg per lane) probed with MPP8 antibody (mAb) (2 µg/ml dilution).

Background

MPP8 (M-Phase Phosphoprotein 8) was originally identified as a phosphoprotein that reacts with MPM2, an antibody that labels mitosis-specific and cell cycle-regulated phosphoproteins. MPP8 contains a single chromodomain that preferentially interacts with nucleosomes enriched for histone H3 lysine 9 methylation but not lysine 4 methylation.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for MPP8 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting