PGC1-β antibody (pAb)

RRID: AB_2793372
Aliases: PPARGC1B, PERC, ERRL1, PGC1B
Catalog No: 39859 Format: 100 µl $500 Buy
Catalog No: 39860 Format: 10 µl $125 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
150 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB*: 1:500 - 1:2,000 dilution

The addition of 0.05% Tween 20 in the blocking buffer and primary antibody incubation buffer is recommended to aid in detection by Western blot. Individual optimization may be required.

*Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Immunogen

This PGC1-β antibody antibody was raised against a peptide within the N-terminal region of human PGC1-β.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

 

PGC1-β antibody tested by Western blot.
Western blot of K-562 nuclear extract (40 µg/lane) probed with PGC1-β antibody (pAb) (1:500 dilution).

Background

PGC1-β (Peroxisome proliferator-activated receptor gamma coactivator 1-beta, PPARGC1B, PERC) is a co-activator of transcription by nuclear receptors and transcription factors. PGC1-β participates in modulating the transcriptional activity of PPAR alpha and gamma, ER alpha, NRF1 and GR. It is likely involved in fat oxidation, non-oxidative glucose metabolism and in the regulation of energy expenditure. Expression of PGC1-β is repressed by saturated fatty acids in skeletal muscle cells and induced by insulin. PGC1-β is down-regulated in type 2 diabetes as well as in pre-diabetics. Allelic variation of PGC1-β may contribute to the pathogenesis of obesity, with the widespread Ala-203 variation being a significant risk factor for the development of this diabetes.

Positive Control

Active Motif's K-562 nuclear extract (Catalog No. 36015) can be used as a positive control for PGC1-β antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting