EZH2 antibody (pAb)

Aliases: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A
Catalog No: 39933 Format: 100 µl $435 Buy
Catalog No: 39934 Format: 10 µl $105 Buy
Catalog No: 39035 Format: 50 µl $250 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
96 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt & Sonication Protocol when preparing nuclear extracts. Visit www.activemotif.com to download the protocol.

Published Applications:
ChIP
IP
IF
WB

Immunogen

This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
EZH2 antibody (pAb) tested by immunoprecipitation.

EZH2 antibody (pAb) tested by Immunoprecipitation.
10 µl of EZH2 antibody was used to immunoprecipitate EZH2 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the EZH2 antibody at a dilution of 1:500.

EZH2 antibody (pAb) tested by Western blot.

EZH2 antibody tested by Western blot.
Detection of EZH2 by Western blot. The analysis was performed using 20 µg of HeLa cell acid extract and EZH2 antibody at a 1:1000 dilution.

Background

EZH2(Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for EZH2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with EZH2 antibody (pAb).

Compatible Secondary Antibodies

Active Motif has developed a variety of high-quality secondary antibodies for most applications, with anti-rabbit and anti-mouse conjugates to a broad line of high-quality fluorescent molecules, as well as horse radish peroxidase (HRP). Visit our Secondary Antibody Conjugates page to find the perfect secondaries for immunofluorescence experiments with EZH2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting