EZH2 antibody (pAb)

RRID: AB_2793397
Aliases: WVS, ENX1, EZH1, KMT6, WVS2, ENX-1, EZH2b, KMT6A
Catalog No: 39933 Format: 100 µl $455 Buy
Catalog No: 39934 Format: 10 µl $110 Buy
Catalog No: 39035 Format: 50 µl $265 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
96 kDa
Reactivity:
Human, Mouse

Applications

ChIP Validated Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB:* 1:500 - 1:2,000 dilution

*Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot.

Published Applications:
ChIP
IP
IF
WB

For EZH2,
we also offer AbFlex® EZH2 Recombinant Antibody (rAb).  For details, see Catalog No. 91159.

Immunogen

This EZH2 antibody was raised against a recombinant fusion protein corresponding to amino acids 1-370 of mouse EZH2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 

EZH2 antibody (pAb) tested by Immunoprecipitation.
10 µl of EZH2 antibody was used to immunoprecipitate EZH2 from 250 µg of HeLa nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the EZH2 antibody at a dilution of 1:500.

EZH2 antibody tested by Western blot.
Detection of EZH2 by Western blot. The analysis was performed using 20 µg of HeLa cell acid extract and EZH2 antibody at a 1:1000 dilution.

Background

EZH2(Enhancer of Zeste homolog 2) is a human homolog of the Drosophila Polycomb-group protein Enhancer of Zeste protein. It contains a SET domain that catalyzes the methylation of histone H3 at lysine 27 (Histone H3 Lys27 monomethylated, dimethylated and trimethylated). Polycomb-group proteins repress gene expression by binding to chromatin and locally altering chromatin structure. EZH2, BMI-1 and Suz12 are present in the PRC2 and PRC3 protein complexes that function as mediators of epigenetic transcriptional silencing. EZH2 associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. EZH2 may play a role in the hematopoietic and central nervous systems. Deregulation of EZH2 is linked to a variety of cancers.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for EZH2 antibody.

Chromatin IP Assays

This antibody has been validated for use in ChIP and/or ChIP-Seq, and can be used with Active Motif's ChIP-IT® High Sensitivity Kit or our magnetic bead-based ChIP-IT® Express Kits. For an overview of all of our ChIP products, please visit our Chromatin IP Page to learn about ChIP products designed for use with EZH2 antibody (pAb).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting