SALL4 antibody (pAb)

RRID: AB_2793408
Aliases: Spalt Like Transcription Factor 4, DRRS, HSAL4, ZNF797, dJ1112F19.1
Catalog No: 39957 Format: 100 µl $445 Buy
Catalog No: 39958 Format: 10 µl $110 Buy

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Antibody Type:
Affinity Purified
Molecular Weight:
142 kDa


Western Blot Validated Immunoprecipitation Validated

Application Notes

Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.


This SALL4 antibody was raised against a synthetic peptide derived from the N-terminus of human SALL4.


Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.



SALL4 antibody (pAb) tested by Immunoprecipitation.
10 µl of SALL4 antibody was used to immunoprecipitate SALL4 from 500 µg of F9 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SALL4 antibody at a dilution of 1:500.

SALL4 antibody (pAb) tested by Western blot.
Nuclear extract of P19 cells (20 μg) probed with SALL4 antibody (1:500).


SALL4 (Sal-like protein 4) is a zinc-finger transcription factor required for the maintenance of embryonic stem cell pluripotency. SALL4 activates the expression of Oct4 and reduction in the levels of SALL4 eliminates the ability of ES cells to self-renew and causes differentiation. SALL4 mutations are associated with the developmental disorders IVIC syndrome and Duane-radial ray syndrome.

Positive Control

Active Motif's P19 nuclear extract (Catalog No. 36074) can be used as a positive control for SALL4 antibody.


Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.


This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • WB = Western Blotting