SALL4 antibody (pAb)

Aliases: Spalt Like Transcription Factor 4, DRRS, HSAL4, ZNF797, dJ1112F19.1
Catalog No: 39957 Format: 100 µl $435 Buy
Catalog No: 39958 Format: 10 µl $105 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
142 kDa
Reactivity:
Mouse

Applications

Western Blot Validated Immunoprecipitation Validated

Application Notes


Validated Applications:
IP: 10 µl per IP
WB: 1:500 - 1:2,000 dilution

Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Immunogen

This SALL4 antibody was raised against a synthetic peptide derived from the N-terminus of human SALL4.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
SALL4 antibody (pAb) tested by immunoprecipitation.

SALL4 antibody (pAb) tested by Immunoprecipitation.
10 µl of SALL4 antibody was used to immunoprecipitate SALL4 from 500 µg of F9 nuclear cell extract (lane 2). 10 µl of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the SALL4 antibody at a dilution of 1:500.

SALL4 antibody (pAb) tested by Western blot.

SALL4 antibody (pAb) tested by Western blot.
Nuclear extract of P19 cells (20 μg) probed with SALL4 antibody (1:500).

Background

SALL4 (Sal-like protein 4) is a zinc-finger transcription factor required for the maintenance of embryonic stem cell pluripotency. SALL4 activates the expression of Oct4 and reduction in the levels of SALL4 eliminates the ability of ES cells to self-renew and causes differentiation. SALL4 mutations are associated with the developmental disorders IVIC syndrome and Duane-radial ray syndrome.

Positive Control

Active Motif's P19 nuclear extract (Catalog No. 36074) can be used as a positive control for SALL4 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting