APOBEC2 antibody (pAb)

Aliases: Apolipoprotein B mRNA-editing enzyme catalytic subunit 2, ARP1, ARCD1
Catalog No: 39985 Format: 100 µl $435 Buy
Catalog No: 39986 Format: 10 µl $105 Buy

Request a quote for a bulk order Bulk Order Button


Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
35 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 - 1:2,000 dilution

Immunogen

This APOBEC2 antibody was raised against a peptide derived from the N-terminal region of human APOBEC2.

Buffer

Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

References

 
APOBEC2 antibody (pAb) tested by Western blot.

APOBEC2 antibody (pAb) tested by Western blot.
Nuclear extract (20 µg) of L6E9 cells (Lane 1) or nuclear extract (20 µg) of C2C12 cells (Lane 2) probed with APOBEC2 antibody at a 1:2,000 dilution.

Background

APOBEC2 (Apolipoprotein B mRNA-editing enzyme catalytic subunit 2) is part of a family of enzymes that edit nucleic acids, possibly changing cytosine to uracil. APOBEC2 expression is regulated by TNF-α via NFκB activation and this suggests a possible role for APOBEC2 in the pathophysiology of hepatic inflammation. APOBEC2 has a low intrinsic cytidine deaminase activity and is also involved in DNA demethylation. APOBEC2 deaminates 5-methylcytosine, followed by base excision repair by Mbd4.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting