PLZF antibody (mAb)

RRID: AB_2793419
Clone: 2A9
Aliases: Promyelocytic Leukaemia Zinc Finger, Zinc Finger And BTB Domain Containing 16, zbtb16
Catalog No: 39987 Format: 100 µg $510 Buy
Catalog No: 39988 Format: 10 µg $125 Buy

Request a quote for a bulk order Request Quote


Product Review Submit a Review


Antibody Type:
Monoclonal
Isotype:
IgG2a
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
90 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated Immunoprecipitation Validated Immunofluorescence Validated

Published Applications

The following applications have been published using this antibody. Unless noted above, Active Motif may not have validated the antibody for use in these applications:

IP
ICC/IF
WB

View publications that use Active Motif products & services here. Enter the product number(s) to see publications & applications that use this antibody.

Immunogen

This PLZF antibody was raised against a recombinant protein corresponding to full-length mouse PLZF with a GST-Tag.

Buffer

PBS pH 7.5 containing 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.

Background

PLZF (Promyelocytic Leukemia Zinc-Finger protein) is a member of the POK (POZ and Krüppel) family of transcriptional repressors. PLZF is expressed in early, but not differentiated, hematopoietic cells, and is required for spermatogonial stem cell maintenance. In addition, PLZF regulates genes involved in cellular proliferation and differentiation (cyclin A2, Myc and Hox genes) and targeted deletion of PLZF in mice results in limb and axial skeleton and limb deformations.

qPCR Primers

Active Motif's Human Negative Control Primer Set 1 (Cat. No. 71001) has been validated for performing qPCR and endpoint PCR when this antibody has been used for ChIP on human samples. For ChIP experiments with this antibody on mouse samples, the Mouse Positive Control Primer Set Hoxc10 (Cat. No. 71019) and Mouse Negative Control Primer Set 3 (Cat. No. 71013) have been validated for both qPCR and endpoint PCR.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting