TBK1 antibody (mAb)

Clone: 108A429
Aliases: NAK, T2K
Catalog No: 40909 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
80 kDa
Reactivity:
Human, Mouse, Rat

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This TBK1 antibody was raised against a peptide corresponding to amino acid residues 563-577 of human TBK1.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
TBK1 antibody (mAb) tested by Western blot.

TBK1 mAb tested by Western blot.
TBK1 detection by Western blot. The analysis of TBK1 was performed using TBK1 mAb at a 2 µg/ml dilution and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TBK1 cDNA (lane 2).

Background

TBK1TANK-binding kinase 1 is a regulator of the NFκB (NFκB p50 and NFκB p65) signaling pathway. NFκB signaling is controlled to a large extent by the sequestration of the NFκB complex in the cytoplasm by its association with one of the IκB family of proteins. IκB is phosphorylated by the IkB Kinase (IKK) complex, resulting in the degradation of IκB and the nuclear translocation of NFκB. TANK is an IκB family member that is specifically recognized and phosphorylated by TBK1, leading to the destruction of TANK and the activation of the NFκB pathway.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for TBK1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting