IRAK-1 antibody (pAb)

Aliases: IRAK, pelle
Catalog No: 40910 Format: 100 µg $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
80 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This IRAK-1 antibody was raised against a peptide corresponding to amino acid residues 700-712 of human IRAK-1.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
IRAK-1 antibody (pAb) tested by Western blot.

IRAK-1 pAb tested by Western blot.
Detection of IRAK-1 by Western blot analysis. The analysis was performed using 10 µg/lane of THP-1 (lane 1) and HeLa (lane 2) nuclear extracts and IRAK-1 pAb at a 2 µg/ml dilution.

Background

IRAK-1Interleukin-1 Receptor-Associated Kinase 1 is one of two serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation to upregulate NFκB (NFκB p50 and NFκB p65) expression.

Positive Control

Active Motif's THP-1 nuclear extract (Catalog No. 36076) can be used as a positive control for IRAK-1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting