RANK antibody (mAb)

Clone: 9A725
Aliases: TNFRSF11A, FEO, OFE, ODFR, OSTS, PDB2, CD265, OPTB7, TRANCER, LOH18CR1
Catalog No: 40917 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
97 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

Published Applications:
WB

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This RANK antibody was raised against a GST-fusion protein containing amino acid residues 326-616 of human RANK.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
RANK antibody (mAb) tested by Western blot.

RANK mAb tested by Western blot.
RANK detection by Western blot. The analysis was performed using 293 cells transfected with RANK cDNA and RANK mAb at a 2 µg/ml dilution. A single protein band of approximate mol. wt. of 97 kDa was detected.

Background

RANK (TNFRSF11A, CD265, PDB2, TRANCER) can interact with various TRAF family proteins (TRAF2, TRAF5), through which this receptor induces the activation of NFκB and MAPK8/JNK. RANK and its ligand are important regulators of the interaction between T cells and dendritic cells. RANK is also an essential mediator for osteoclast and lymph node development. Studies of the mouse counterpart suggest that this receptor directly mediates the osteoprotegerin (OPG) ligand (OPGL)-induced oteoclastogenesis in osteoclast precursor cells.

Positive Control

Extracts from 293 cells transfected with RANK cDNA can be used as a positive control for RANK antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting