TRAF5 antibody (mAb)

RRID: AB_2793439
Clone: 55A219
Aliases: RNF84, MGC:39780
Catalog No: 40920 Format: 100 µg $460 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
66 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This TRAF5 antibody was raised against a fusion protein corresponding to amino acid residues 77 to 186 of human TRAF5.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

TRAF5 mAb tested by Western blot.
TRAF5 detection by Western blot. The analysis was performed using 10 µg HeLa nuclear extract and TRAF5 mAb at a 2 µg/ml dilution.

Background

TRAF5 (TNF receptor-associated factor 5, RNF84) scaffold protein is a member of the tumor necrosis factor receptor-associated factor (TRAF) protein family and contains a meprin and TRAF homology (MATH) domain, a RING-type zinc finger, and two TRAF-type zinc fingers. TRAF proteins are associated with, and mediate signal transduction from, members of the TNF receptor superfamily. TRAF5 is one of the components of a multiple protein complex that binds to tumor necrosis factor (TNF) receptor cytoplasmic domains and mediates TNF-induced activation. TRAF5 mediates activation of NFκB (NFκB p50 & NFκB p65) and probably JNK. It also appears to be involved in apoptosis.

Positive Control

Active Motif's HeLa nuclear extract (Catalog No. 36010) can be used as a positive control for TRAF5 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting