DR6 antibody (pAb)

Aliases: TNFRSF21, CD358, BM-018
Catalog No: 40927 Format: 100 µg $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
66 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This DR6 antibody was raised against a cytoplasmic domain corresponding to amino acid residues 371-655 of human DR6.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
DR6 antibody (pAb) tested by Western blot.

DR6 pAb tested by Western blot analysis.
Western blot analysis of 30 µg of total cell lysate from Jurkat (lane 1), HeLa (lane 2), and Daudi (lane 3) cells tested at 2 µg/ml.

Background

DR6 (also known as Tumor Necrosis Factor Receptor Superfamily, member 21, TNFRSF21) is a member of the TNF-receptor superfamily. This receptor interacts with TRADD to mediate signal transduction of TNF receptors. It may activate NFκB (NFκB p50 and NFκB p65) and MAPK8/JNK and induce apoptosis.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for DR6 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting