Caspase-9 antibody (pAb) (Pro-form)

RRID: AB_2793446
Aliases: CASP9
Catalog No: 40931 Format: 100 µg $295 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
46 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:1,000 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This Caspase-9 antibody (Pro-form) was raised against a peptide corresponding to amino acid residues 299 -318 of human Pro-Caspase-9.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

Caspase-9 pAb (Pro-form) tested by Western blot.
Caspase-9 detection by Western blot. The analysis was performed using 50 µg HeLa whole-cell extract and Caspase-9 pAb (Pro-form) at a 1:1,000 dilution.

Background

Caspase-9 (CASP9 or apoptosis-related cysteine peptidase 9) (Pro-form) is involved in the activation cascade of caspases responsible for apoptosis execution. Caspase-9 is processed by caspase APAF1, which is thought to be one of the earliest events in the caspase activation cascade. Caspase-9 is then involved in processing of Caspase-3.

Positive Control

Active Motif's HeLa whole-cell extract (Catalog No. 40050) can be used as a positive control for Caspase-9 antibody (Pro-form).

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting