Caspase-14 antibody (mAb)

Clone: 70A1426
Aliases: CASP14
Catalog No: 40932 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
30 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This Caspase-14 antibody was raised against a synthetic peptide corresponding to amino acid residues 2-18 of mouse Caspase-14.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
Caspase-14 antibody (mAb) tested by Western blot.

Caspase-14 mAb tested by Western blot.
Caspase-14 detection by Western blot. The analysis was performed using 10 µg of mouse spleen cell extract and Caspase-14 mAb at a 2 µg/ml dilution.

Background

Caspase-14 (CASP14 or apoptosis-related cysteine peptidase 14) is thought to be a non-apoptotic caspase involved in epidermal differentiation. Caspase-14 has been shown to be processed and activated by Caspase-8 and Caspase 10 in vitro, and also by TNF-Related Apoptosis-Inducing Ligand (TRAIL) in vivo.

Positive Control

Extracts from mouse spleen can be used as a positive control for Caspase-14 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting