D4-GDI antibody (mAb)

Clone: 97A1015
Aliases: ARHGDIB, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2
Catalog No: 40941 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
23 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This D4-GDI antibody was raised against a synthetic peptide corresponding to amino acid residues in the Fas-induced cleavage site of human D4-GDI.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
D4-GDI antibody (mAb) tested by Western blot.

D4-GDI mAb tested by Western blot.
Detection of D4-GDI by Western blot analysis. HL-60 cells were treated for different time periods with 10 ng/ml of Fas monoclonal antibody. Harvested cells were lysed, resolved by SDS-PAGE and transferred onto a PVDF membrane. The membrane was incubated with the D4-GDI mAb and immunoreactivity was detected by chemiluminescence. The data shows that the D4-GDI antibody specifically detects the 23 kDa cleaved form of D4-GDI in a time-sensitive manner.

Background

D4-GDI (Rho GDP dissociation inhibitor beta or ARHGDIB) plays an important role in the activation of the superoxide (O2-)-generating NADPH oxidase of phagocytes. The mechanisms involving cleavage of D4-GDI (Rho GDP) with apoptosis are not presently known. However, activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting