D4-GDI antibody (mAb)

RRID: AB_2793453
Clone: 97A1015
Aliases: ARHGDIB, D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2
Catalog No: 40941 Format: 100 µg $460 Buy

Request a quote for a bulk order Request Quote


Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
23 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This D4-GDI antibody was raised against a synthetic peptide corresponding to amino acid residues in the Fas-induced cleavage site of human D4-GDI.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

D4-GDI mAb tested by Western blot.
Detection of D4-GDI by Western blot analysis. HL-60 cells were treated for different time periods with 10 ng/ml of Fas monoclonal antibody. Harvested cells were lysed, resolved by SDS-PAGE and transferred onto a PVDF membrane. The membrane was incubated with the D4-GDI mAb and immunoreactivity was detected by chemiluminescence. The data shows that the D4-GDI antibody specifically detects the 23 kDa cleaved form of D4-GDI in a time-sensitive manner.

Background

D4-GDI (Rho GDP dissociation inhibitor beta or ARHGDIB) plays an important role in the activation of the superoxide (O2-)-generating NADPH oxidase of phagocytes. The mechanisms involving cleavage of D4-GDI (Rho GDP) with apoptosis are not presently known. However, activation of the Jun N-terminal kinase, a regulator of apoptosis, may be one of the mechanisms.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting