TLR3 antibody (mAb)

Clone: 40C1285
Aliases: CD283, IIAE2
Catalog No: 40952 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
120 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This TLR3 antibody was raised against a KLH-conjugated synthetic peptide corresponding to human TLR3.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
TLR3 antibody (mAb) tested by Western blot.

TLR3 mAb tested by Western blot.
TLR3 detection by Western blot. The analysis of TLR3 was performed using TLR3 mAb and nuclear extract from 293 cells (lane 1) and nuclear extract from 293 cells that had been transfected with human TLR3 cDNA (lane 2).

Background

TLR3 – Toll-Like receptor 3 is a member of the Toll-like receptor (TLR) family that plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize specific molecular features associated with pathogens, and mediate the production of cytokines necessary for the development of effective immunity. Many of the TLRs heterodimerize with each other to modulate receptor function. TLR3 expression is increased after exposure to Gram-negative bacteria and to an even greater extent in response to Gram-positive bacteria. TLR3 forms a homodimer and recognizes viral double stranded RNA (dsRNA).

Positive Control

Whole-cell extract from human intestine can be used as a positive control for TLR3 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting