DAP-5 antibody (mAb)

Clone: 39C534
Aliases: EIF4G2, P97, AAG1, DAP5, NAT1
Catalog No: 40964 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
97 kDa
Reactivity:
Human, Mouse

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This DAP-5 antibody was raised against a synthetic peptide corresponding to amino acid residues 796-812 of human DAP-5.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
DAP-5 antibody (mAb) tested by Western blot.

DAP-5 mAb tested by Western blot.
DAP-5 detection by Western blot. The analysis was performed using 15 µg of Jurkat whole-cell extract and the DAP-5 mAb at a 1 µg/ml dilution.

Background

DAP-5 (eIF4G2 or eukaryotic translation initiation factor 4 gamma 2) shares similarity with the C-terminal region of eIF4G, which contains the binding sites for eIF4A and eIF3. eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. DAP-5 appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting