p73 antibody (mAb)

Clone: 5B429
Aliases: TP73
Catalog No: 40972 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
73 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1:500 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This p73 antibody was raised against a full-length protein corresponding to human p73. This p73 antibody reacts with an epitope that is located on the N-terminal region of human p73 and does not cross react with p53. The p73 protein may run at 63 kDa in some cell lines due to post-transcriptional modifications.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
p73 antibody (mAb) tested by Western blot.

p73 mAb tested by Western blot.
p73 monoclonal antibody used at a 1:500 dilution against 10 µg of transfected cell lysate.

Background

p73 is a p53-related tumor protein, with some structural resemblance to p53, and is therefore considered a tumor-suppressor protein. p73 has numerous isoforms arising from alternative splicing, resulting in 6 different C-termini. Overexpression of p73 promotes growth arrest and/or apoptosis in a manner similar to that seen with p53.

Positive Control

Extracts from cells transfected with p73 can be used as a positive control for p73 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting