p73 antibody (mAb)

RRID: AB_2793465
Clone: 5B429
Aliases: TP73
Catalog No: 40972 Format: 100 µg $460 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1, k
Purification:
Protein G Chromatography
Host:
Mouse
Molecular Weight:
73 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes

Applications Validated by Active Motif:
WB: 1:500 dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.

Immunogen

This p73 antibody was raised against a full-length protein corresponding to human p73. This p73 antibody reacts with an epitope that is located on the N-terminal region of human p73 and does not cross react with p53. The p73 protein may run at 63 kDa in some cell lines due to post-transcriptional modifications.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

 

p73 mAb tested by Western blot.
p73 monoclonal antibody used at a 1:500 dilution against 10 µg of transfected cell lysate.

Background

p73 is a p53-related tumor protein, with some structural resemblance to p53, and is therefore considered a tumor-suppressor protein. p73 has numerous isoforms arising from alternative splicing, resulting in 6 different C-termini. Overexpression of p73 promotes growth arrest and/or apoptosis in a manner similar to that seen with p53.

Positive Control

Extracts from cells transfected with p73 can be used as a positive control for p73 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Guarantee

This product is guaranteed for 12 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • ChIP = ChIP Sequencing;
  • CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
  • CUT&Tag = Cleavage Under Targets and Tagmentation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • NEU = Neutralizing;
  • WB = Western Blotting