p73ΔN antibody (mAb)
|Catalog No: 40973||Format: 100 µg||$460||Buy|
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Applications Validated by Active Motif:
WB: 1 - 2 µg/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
This p73DN antibody was raised against a peptide corresponding to amino acid residues 2-13 of human p73ΔN. This antibody does not cross react with full-length p73.
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
p73ΔN is a member of the p53 protein family which includes p53 as well as p63. Whereas p53 transactivates the retinoblastoma gene, p63 and p73 induce the cyclin-dependent kinase inhibitor p57 to maintain RB1 in an active, hypophosphorylated state. p73ΔN inhibits the functions of the p53 family in differentiation control, prevents myogenic differentiation, and enables cooperating oncogenes to transform myoblasts to tumorigenicity. p73ΔN is frequently overexpressed in rhabdomyosarcoma and is essential for tumor progression in vivo. These findings establish differentiation control as a key tumor suppressor activity of the p53 family.
Extracts from cells transfected with p73ΔN cDNA can be used as a positive control for p73ΔN antibody.
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
This product is guaranteed for 12 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
- ChIP = Chromatin Immunoprecipitation;
- ChIP = ChIP Sequencing;
- CUT&RUN = Cleavage Under Targets and Release Using Nuclease;
- CUT&Tag = Cleavage Under Targets and Tagmentation;
- DB = Dot Blot;
- ELISA = Enzyme-linked Immunosorbent Assay;
- EMSA = Electrophoretic Mobility Shift Assay
- FC = Flow Cytometry;
- ICC = Immunocytochemistry;
- IF = Immunofluorescence;
- IHC = Immunohistochemistry;
- IP = Immunoprecipitation;
- MeDIP = Methyl-DNA Immunoprecipitation;
- NEU = Neutralizing;
- WB = Western Blotting