ATF-2 phospho Thr71 antibody (mAb)

Clone: 103C411
Aliases: HB16, CREB2, TREB7, CREB-2, CRE-BP1
Catalog No: 40975 Format: 100 µg $435 Buy

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Antibody Type:
Monoclonal
Isotype:
IgG1
Purification:
Affinity Purified
Host:
Mouse
Molecular Weight:
61 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This ATF-2 phospho Thr71 antibody was raised against a synthetic peptide containing phosphorylated threonine at position 71 corresponding to human Phosphorylated-ATF-2.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
ATF-2 phospho Thr71 antibody (mAb) tested by Western blot.

ATF-2 phospho Thr71 mAb tested by Western blot.
ATF-2 (phospho) detection by Western blot. Detection of ATF-2 phosphorylation in HeLa cell extract with ATF-2 phospho Thr71 mAb.
    Lane A: Untreated HeLa nuclear extract;
    Lane B: HeLa nuclear extract treated with TNF-α for 1 hour.

Background

ATF-2 (activating transcription factor 2) transcription factor is a member of the leucine-zipper family of proteins and was previously known as cAMP responsive element binding protein 2 (or CREB2). ATF 2 is a transcriptional activator that binds the cAMP-responsive element (CRE) and interacts with c-Jun to stimulate CRE transcription. This protein is also capable of acetylating Histone H2B and Histone H4 in vitro as a histone acetyltransferase (HAT).

Positive Control

Active Motif's HeLa nuclear extract (TNF-α stimulated) (Catalog No. 40210) can be used as a positive control for ATF-2 phospho Thr71 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting