Rad1 antibody (pAb)

Aliases: RAD1 Checkpoint DNA Exonuclease, Cell Cycle Checkpoint Protein Hrad1, DNA Repair Exonuclease REC1
Catalog No: 40978 Format: 100 µg $435 Buy

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Antibody Type:
Polyclonal
Isotype:
IgG
Purification:
Affinity Purified
Host:
Rabbit
Molecular Weight:
66 kDa
Reactivity:
Human

Applications

Western Blot Validated

Application Notes


Validated Applications:
WB: 1 - 2 µg/ml dilution

For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all Blotto solutions may also reduce background. Individual optimization may be required.

Immunogen

This Rad1 antibody was raised against a synthetic peptide corresponding to amino acid residues 133-148 of human Rad1.

Buffer

PBS containing 0.02% sodium azide. Sodium azide is highly toxic.

References

 
Rad1 antibody (pAb) tested by Western blot.

Rad1 pAb tested by Western blot.
Rad1 detection by Western blot. hRad1 rabbit pAb was diluted at 2 µg/ml (lane 1) and 1 µg/ml (lane 2) and tested against 10 µg of 293 nuclear extract by Western blot. A single band at 66 kDa is detected.

Background

Rad1 (REC1) is a component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the Rad17-replication factor C (RFC) clamp loader complex. Rad1 then acts as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3′-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. Rad1 isoform 1 possesses 3′-5′ double-stranded DNA exonuclease activity.

Positive Control

Active Motif's 293 nuclear extract (Catalog No. 36033) can be used as a positive control for Rad1 antibody.

Storage

Some products may be shipped at room temperature. This will not affect their stability or performance. Upon receipt, unconjugated antibodies may be stored at -20°C for up to 2 years. Fluorophore- & enzyme-conjugated antibodies should be stored at 4°C. Fluorophore-conjugated antibodies should be protected from light. Keep reagents on ice when not in storage; to avoid repeated freeze/thaw cycles, we recommend aliquoting items that will be stored frozen into single-use fractions prior to freezing.

Guarantee

This product is guaranteed for 6 months from date of receipt.

This product is for research use only and is not for use in diagnostic procedures.

Application Key

  • ChIP = Chromatin Immunoprecipitation;
  • DB = Dot Blot;
  • ELISA = Enzyme-linked Immunosorbent Assay;
  • EMSA = Electrophoretic Mobility Shift Assay
  • FC = Flow Cytometry;
  • ICC = Immunocytochemistry;
  • IF = Immunofluorescence;
  • IHC = Immunohistochemistry;
  • IP = Immunoprecipitation;
  • MeDIP = Methyl-DNA Immunoprecipitation;
  • TR-FRET = Time-Resolved Fluorescence Resonance Energy Transfer;
  • WB = Western Blotting